YOU WHO GOT A LAB  (THE CRBCM CAN'T, CRBCM DOES NOT HAVE  POLITICAL BACK BONE, WE ARE MUZZLED, POLITICALLY STOPPED FOR DOING RESEARCH, SO WE GIVE YOU THE CUES)
GO GET THE CURE FOR US!

USE shRNA TO SILENCE PI3K IN MELANOMA
OR RESTORE PTEN FUNCTION!

Small hairpin RNA

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Lentiviral delivery of shRNA and the mechanism of RNA interference in mammalian cells.

A small hairpin RNA or short hairpin RNA (shRNA) is a sequence of RNA that makes a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi). Expression of shRNA in cells is typically accomplished by delivery of plasmids or through viral or bacterial vectors. The promoter choice is essential to achieve robust shRNA expression. At first, polymerase III promoters such as U6 and H1 were used; however, these promoters lack spatial and temporal control.^[1] As such, there has been a shift to using polymerase II promoters to regulate expression of shRNA. shRNA is an advantageous mediator of RNAi in that it has a relatively low rate of degradation and turnover. However, shRNA is disadvantageous in that it requires use of an expression vector which can pose safety concerns.