Activity at CRBCM

Today we have concluded a meeting with DR Zhang and (UTEP) and DR Bryan (Texas Tech) on future projects.  We have discussed our collaborative efforts on several  projects, and discussed to use "gene interference" techniques to affect several genes and see whether we can affect several pathways for the cure of cancer.
Several critical areas:
ie.  Could blocking PMS2 (MLH-1  or MLH1 protein ) increase the efficiency of 5-FU in Colon cancer?
ie. Could activation of ARF amplify MDM2 with increased proteosomal degration of P53 in certain Sarcoma?
ie. Could a genetically engineered AATCC Nucleotide set keep Telomeres busy to increase Apoptosis?
ie. Could genetic instability induced by inactivation of telomerase increase radiation sensitivity and response to certain chemotherapy agents?
ie. Examining the role of RPTPs in Alzheimer dementia
ie. Blocking the Farnesyl to delocalize RAS in lung cancers
ie. Blocking Phospholipase C in PIK3 driven cancers?

The meeting was concluded with a tour of the Laboratory.
Our work is cut out, CRBCM, is still working hard for the cure, no stone will stay unturned!

Yes, don't forget the TELOMERES!

• October 2013
• > Vannier et al., 342 (6155): 239-242

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 WATCH THIS ONE!

RTEL1 Is a Replisome-Associated Helicase That Promotes Telomere and Genome-Wide Replication

1. Jean-Baptiste Vannier¹,*,
2. Sumit Sandhu²,*,
3. Mark IR. Petalcorin¹,
4. Xiaoli Wu²,
5. Zinnatun Nabi²,
6. Hao Ding²,³,^†,
7. Simon J. Boulton¹,^†

+ Author Affiliations

1. ¹DNA Damage Response laboratory, London Research Institute, Cancer Research UK, Clare Hall, South Mimms EN6 3LD, UK.
2. ²Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3E 3J7, Canada.
3. ³Manitoba Institute of Child Health, Winnipeg, Manitoba, R3E 3P4, Canada.

1. ↵†Corresponding author. E-mail: dingh@cc.umanitoba.ca (H.D.); simon.boulton@cancer.org.uk (S.J.B.)

1. ↵* These authors contributed equally to this work.

• Abstract
• Editor's Summary

Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles telomere loops (T loops) and suppresses telomere fragility to maintain the integrity of chromosome ends. We established that RTEL1 also associates with the replisome through binding to proliferating cell nuclear antigen (PCNA). Mouse cells disrupted for the RTEL1-PCNA interaction (PIP mutant) exhibited accelerated senescence, replication fork instability, reduced replication fork extension rates, and increased origin usage. Although T-loop disassembly at telomeres was unaffected in the mutant cells, telomere replication was compromised, leading to fragile sites at telomeres. RTEL1-PIP mutant mice were viable, but loss of the RTEL1-PCNA interaction accelerated the onset of tumorigenesis in p53-deficient mice. We propose that RTEL1 plays a critical role in both telomere and genome-wide replication, which is crucial for genetic stability and tumor avoidance.
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PLEASE GO TO THE FULL ARTICLE,
GOOD JOB FOR THESE SCIENTISTS!

STILL LOOKING FOR NEW TARGETS FOR THE CURE OF CANCER?

WELL I GIVE YOU THESE TARGETS DEEP IN THE NUCLEUS

1. Esap 1
2. Esw-1
3. Esc-2 and 8
and the mighty NPT-1 gene

Here you are at the heart of cell survival
as you touch on Histone Deacetylase, gene silencing (Including Ribosomal genes or RNA) and Telomeres.
and I gave you a clue, Antibiotic from fungi can help!
The cure is at the door, open it!

FACTORS BEING MONITORED IN THE WELLNESS PROGRAM (part II)

1. AGE
Because with age comes all the trouble.  Age is a relative thing.  At what age do we start monitoring things?
And 40 years of age appears the most thought of in Western Societies.  Mammograms are started and 45 years is a risk factor for men who then ae being monitor for cholesterol.  The natural or true age is linked to genetic and environmental factors.  Age is linked to the length of Telomeres and to Senescence.  Telomeres are these youth protector molecules which keep being clipped and shortened  at each cell cycle until the DNA is exposed to Endonucleases (if you get my drift!).  Sometime we can fool it and make it a variable by exercising, and then it plays catch-up with you later.  Some time, it is not so much of a variable, it is just there to be lived with...Suffice is to say, when you are young you feel invincible, protected.  Being old, well, you have a "Risk factor" .  Cancer does not like to be old, and works hard to preserve or elongate its Telomeres.  This is an area of Target therapy.

2.  Cholesterol, we spoke about yesterday-see WELLNESS PART I

3.  WEIGHT MANAGEMENT WE WILL SPEAK ABOUT NEXT
4.  BLOOD PRESSURE, Hb A1C FOR DIABETIC
5. THYROID FUNCTION (THYROID STIMULATING HORMONE LEVEL)
6. BNP

Brain natriuretic peptide - Wikipedia, the free encyclopedia

en.wikipedia.org/wiki/Brain_natriuretic_peptide

Basic natriuretic peptide (BNP), now known as B-type natriuretic peptide (also BNP) or GC-B, is a 32 amino acid polypeptide secreted by the ventricles of the ...

7.RENAL FUNCTION AND CREATININE
8. HEMOGLOBIN LEVEL
9. C-REACTIVE PROTEIN STATUS    (AND ANTI NUCLEAR ANTIBODY STATUS)
10. FEV 1 (OR WHETHER YOU FEEL TIRED AND SHORT OF BREATH)
11. OXYGEN SATURATION OR SLEEP APNEA PRESENCE
12. ALBUMIN LEVEL  (LOWER THAN 3.5g)
13. MENTAL STATUS / ANXIETY
14. PROTEINURIA
15. ADHESION TO PREVENTION AND IMMUNIZATION
(FLU-SHOT, PNEUMONIA AND NOW SHINGLE SHOT, ANNUAL OPHTHALMO VISIT,MAMMOGRAM,COLONOSCOPY,BONE DENSITY MONITOR, SMOKING CESSATION AND AVOIDANCE OF ALCOHOLI SM)


THESE 15 FACTORS SUM UP YOUR HEALTH.  THIS IS THE REPORT CARD YOU SHOULD GET FROM YOUR DOCTOR WITH EVERY VISIT!

WE WILL DISCUSS THEM AS WE GO ALONG