AQUA TECHNOLOGY-AUTOMATED QUANTITATIVE ANALYSIS
IT IS A QUANTITATIVE IMMUNOPHENOTYPING TECHNIQUE
DISCUSSED BY EXPERTS (ASCO/CAP GUIDELINES AND NCCN TASKFORCE) MOST APPROPRIATE FOR SAMPLE WITH 'LOW NUCLEAR STAINING".  it has proven itself for measurement of ER and PR positivity in breast cancers!
The Cutt-off is 5.2 for determining receptor status
AQUA combines immunofluorescence and automated image technology.

NCCN
- ER and PR negative tumor status should be retested if tumor histology is lobular,tubular or mucinous because these types of tumors are rarely negative
-Also retest if tumors are GRADE1 since these are rarely negative
-Retesting is indicated at Diagnosis and at Relapse
-The option of endocrine therapy for ER&PR negative tumors does not depend on level of hormone Receptor Expression.

With this technique,
the cut off point for positive HER-2 is 572
less than 572, no HER-2 expression
more than 572, positive HER-2 

(Material provided by Genoptix )