INTERESTING OBSERVATIONS

?Is it true that Azacitine will work better in warm conditions.
?will Azacidine work better in DNMT1 mutated cells
?is DNMT1 critical to the Polycomb/trithorax complex function
De-methylating without disabling this polycomb function mitigates the impact of demethylation because of memory issues!  The cell seems to remember its status before de-methylation, you need to knock down DNMT1to potentiate the effect of 5-Azacitidine!  May be adding Retinoic Acid?

COMBINATION OF ATRA WITH 5-AZACITIDINE?

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Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system.

Post WS

KIM " This activity was augmented by histone deacetylase inhibition. These findings provide evidence of epigenetic dysregulation of estrogen receptor beta in atherosclerosis and vascular aging. We suggest that focal epigenetic changes in estrogen receptor beta contribute to the development of atherosclerosis and vascular aging." 
POST MENOPAUSAL WOMEN SEE A DROP OF ESTROGEN, A METHYLATION OR THE GENE MAKING THE ESTROGEN RECEPTOR IS A PROGRAMMED EVENT, WILL DYSREGULATION OF THE POLYCOMB CHANGE THAT? OR IS-IT MUTATION OF THE DNMT1 AGAIN ENOUGH?

MicroRNA-152 mediates DNMT1-regulated DNA methylation in the estrogen receptor α gene.

Wang Y ET AL

WHY LUPUS IS ACTIVE IN WOMEN AGE 15-44

IT HAS TO DO WITH THIS!

CESCHIN ET AL
"Multiple signaling pathways ultimately modulate the epigenetic information embedded in the chromatin of gene promoters by recruiting epigenetic enzymes. We found that, in estrogen-regulated gene programming, the acetyltransferase CREB-binding protein (CBP) is specifically and exclusively methylated by the coactivator-associated arginine methyltransferase (CARM1) in vivo. CARM1-dependent CBP methylation and p160 coactivators were required for estrogen-induced recruitment to chromatin targets. Notably, methylation increased the histone acetyltransferase (HAT) activity of CBP and stimulated its autoacetylation. Comparative genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) studies revealed a variety of patterns by which p160, CBP, and methyl-CBP (meCBP) are recruited (or not) by estrogen to chromatin targets. Moreover, significant target gene-specific variation in the recruitment of (1) the p160 RAC3 protein, (2) the fraction of a given meCBP species within the total CBP, and (3) the relative recruitment of different meCBP species suggests the existence of a target gene-specific “fingerprint” for coregulator recruitment."

BELIEVE ME, ESTROGEN INCREASES GENE METHYLATION
PEGGY SUGGESTED OOPHORECTOMY AS MEANS TO CONTROL BAD LUPUS  IN WOMEN OF REPRODUCTIVE AGE WHO HAVE HAD THEIR CHILDREN !? WHAT DO YOU THINK?