MORE ABOUT 5-AZACITIDINE

Be careful though, when you give an hypomethylating agent, do not expect that the agent is de-methylating everywhere, some molecule end up being methylated, this is called Reverse Methylation.  There seem to me a random (or not) reorganization of the methylation.

Moreau
"Exposure of cells to histone deacetylase inhibitors, or to the demethylating agent 5-aza-2′-deoxycytidine, revealed that HLA-G gene transcription is inhibited by DNA methylation. Reversal of methylation-mediated repression may directly induce HLA-G cell-surface expression, supporting the idea that HLA-G might be activated by such a mechanism during malignancy, inflammation, and allogenic reactions"

what is the meaning of this
is this point to limitation of this drug activity
should we be checking for HLA-G
more work is ahead I guess!